pfa6a his3mx6 plasmid Search Results


92
Addgene inc gal1 induced alleles
Gal1 Induced Alleles, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc his3mx6 cassette
His3mx6 Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfa6a+his3mx6+plasmid/pFA6a-his3MX6+(Plasmid+%2333139)/pm40975060-362-14-20
Average 93 stars, based on 1 article reviews
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Addgene inc pfa6a 3ha his3mx
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Addgene inc pfa6a his3mx6
Pfa6a His3mx6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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Addgene inc pfa6a his3mx6 pgal1
Pfa6a His3mx6 Pgal1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc pacyc yham
(A) Exploded view of the GMPS. Layers were adhered with double-sided 50-μm 3M adhesive tape. (B) Full-channel brightfield image of Caco-2 cells in GMPS after 10 days under flow. Multiple images were stitched together to create the tiled image. (C) Immunofluorescent staining of Caco-2 cells after 10 days in GMPS. Immunostaining for ZO-1 (red) and Hoechst (cyan). The image is a z stack maximum intensity projection. (D) Schematic of strain development and different promoter-gene combinations to control H 2 S titration. Different strength promoters lac, BAD, or T7 were used to control the expression of the desulfidases ( cdl or <t>yhaM</t> ) or the putative cysteine transporter, yhaO . DecR controls the yhaOM operon; yhaO is a putative cysteine transporter, and yhaM is a cysteine desulfidase. decR was deleted from MG1655 and used as the chassis strain moving forward. (E) Schematic of GMPS co-culture with engineered microbes and endpoint analyses. (D) and (E) were created with biorender.com .
Pacyc Yham, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc pu6 crrna ms2
(A) Exploded view of the GMPS. Layers were adhered with double-sided 50-μm 3M adhesive tape. (B) Full-channel brightfield image of Caco-2 cells in GMPS after 10 days under flow. Multiple images were stitched together to create the tiled image. (C) Immunofluorescent staining of Caco-2 cells after 10 days in GMPS. Immunostaining for ZO-1 (red) and Hoechst (cyan). The image is a z stack maximum intensity projection. (D) Schematic of strain development and different promoter-gene combinations to control H 2 S titration. Different strength promoters lac, BAD, or T7 were used to control the expression of the desulfidases ( cdl or <t>yhaM</t> ) or the putative cysteine transporter, yhaO . DecR controls the yhaOM operon; yhaO is a putative cysteine transporter, and yhaM is a cysteine desulfidase. decR was deleted from MG1655 and used as the chassis strain moving forward. (E) Schematic of GMPS co-culture with engineered microbes and endpoint analyses. (D) and (E) were created with biorender.com .
Pu6 Crrna Ms2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc viruses aav5 ef1α dio eyfp addgene
(A) Exploded view of the GMPS. Layers were adhered with double-sided 50-μm 3M adhesive tape. (B) Full-channel brightfield image of Caco-2 cells in GMPS after 10 days under flow. Multiple images were stitched together to create the tiled image. (C) Immunofluorescent staining of Caco-2 cells after 10 days in GMPS. Immunostaining for ZO-1 (red) and Hoechst (cyan). The image is a z stack maximum intensity projection. (D) Schematic of strain development and different promoter-gene combinations to control H 2 S titration. Different strength promoters lac, BAD, or T7 were used to control the expression of the desulfidases ( cdl or <t>yhaM</t> ) or the putative cysteine transporter, yhaO . DecR controls the yhaOM operon; yhaO is a putative cysteine transporter, and yhaM is a cysteine desulfidase. decR was deleted from MG1655 and used as the chassis strain moving forward. (E) Schematic of GMPS co-culture with engineered microbes and endpoint analyses. (D) and (E) were created with biorender.com .
Viruses Aav5 Ef1α Dio Eyfp Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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Addgene inc dna template pfa6a link 3xflag sphis5
(A) Exploded view of the GMPS. Layers were adhered with double-sided 50-μm 3M adhesive tape. (B) Full-channel brightfield image of Caco-2 cells in GMPS after 10 days under flow. Multiple images were stitched together to create the tiled image. (C) Immunofluorescent staining of Caco-2 cells after 10 days in GMPS. Immunostaining for ZO-1 (red) and Hoechst (cyan). The image is a z stack maximum intensity projection. (D) Schematic of strain development and different promoter-gene combinations to control H 2 S titration. Different strength promoters lac, BAD, or T7 were used to control the expression of the desulfidases ( cdl or <t>yhaM</t> ) or the putative cysteine transporter, yhaO . DecR controls the yhaOM operon; yhaO is a putative cysteine transporter, and yhaM is a cysteine desulfidase. decR was deleted from MG1655 and used as the chassis strain moving forward. (E) Schematic of GMPS co-culture with engineered microbes and endpoint analyses. (D) and (E) were created with biorender.com .
Dna Template Pfa6a Link 3xflag Sphis5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Exploded view of the GMPS. Layers were adhered with double-sided 50-μm 3M adhesive tape. (B) Full-channel brightfield image of Caco-2 cells in GMPS after 10 days under flow. Multiple images were stitched together to create the tiled image. (C) Immunofluorescent staining of Caco-2 cells after 10 days in GMPS. Immunostaining for ZO-1 (red) and Hoechst (cyan). The image is a z stack maximum intensity projection. (D) Schematic of strain development and different promoter-gene combinations to control H 2 S titration. Different strength promoters lac, BAD, or T7 were used to control the expression of the desulfidases ( cdl or yhaM ) or the putative cysteine transporter, yhaO . DecR controls the yhaOM operon; yhaO is a putative cysteine transporter, and yhaM is a cysteine desulfidase. decR was deleted from MG1655 and used as the chassis strain moving forward. (E) Schematic of GMPS co-culture with engineered microbes and endpoint analyses. (D) and (E) were created with biorender.com .

Journal: Cell reports

Article Title: Engineered bacteria titrate hydrogen sulfide and induce concentration-dependent effects on the host in a gut microphysiological system

doi: 10.1016/j.celrep.2023.113481

Figure Lengend Snippet: (A) Exploded view of the GMPS. Layers were adhered with double-sided 50-μm 3M adhesive tape. (B) Full-channel brightfield image of Caco-2 cells in GMPS after 10 days under flow. Multiple images were stitched together to create the tiled image. (C) Immunofluorescent staining of Caco-2 cells after 10 days in GMPS. Immunostaining for ZO-1 (red) and Hoechst (cyan). The image is a z stack maximum intensity projection. (D) Schematic of strain development and different promoter-gene combinations to control H 2 S titration. Different strength promoters lac, BAD, or T7 were used to control the expression of the desulfidases ( cdl or yhaM ) or the putative cysteine transporter, yhaO . DecR controls the yhaOM operon; yhaO is a putative cysteine transporter, and yhaM is a cysteine desulfidase. decR was deleted from MG1655 and used as the chassis strain moving forward. (E) Schematic of GMPS co-culture with engineered microbes and endpoint analyses. (D) and (E) were created with biorender.com .

Article Snippet: pACYC- yhaM , This study, see , Addgene Plasmid #207650.

Techniques: Adhesive, Staining, Immunostaining, Control, Titration, Expressing, Co-Culture Assay

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Engineered bacteria titrate hydrogen sulfide and induce concentration-dependent effects on the host in a gut microphysiological system

doi: 10.1016/j.celrep.2023.113481

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: pACYC- yhaM , This study, see , Addgene Plasmid #207650.

Techniques: Virus, Recombinant, Plasmid Preparation, Software

Journal: Cell reports

Article Title: Engineered bacteria titrate hydrogen sulfide and induce concentration-dependent effects on the host in a gut microphysiological system

doi: 10.1016/j.celrep.2023.113481

Figure Lengend Snippet:

Article Snippet: pACYC- yhaM , This study, see , Addgene Plasmid #207650.

Techniques: Control